The prevalence of the metabolic syndrome (MS) is rapidly increasing all over the world.
Consequently, there is an urgent need for more effective intervention strategies. Both animal
and human studies indicate that lipid oversupply to skeletal muscle can result in insulin
resistance, which is one of the characteristics of the MS. C57BL/6J mice were fed a low-fat
(10 kcal%) palm oil diet or a high-fat (45 kcal%; HF) palm oil diet for 3 or 28 days. By
combining transcriptomics with protein and lipid analyses we aimed to better understand the …

METHODS Animals and study protocol. Male C57BL/6J mice were obtained from Charles
River (Maastricht, The Netherlands) at 7 wk of age. The mice were fed a standard growth
diet [RM3 (E) DU; Special Diet Services, Witham, UK] for 2 wk prior to switching to the low-fat
(LF) run-in diet for 3 wk. Following the run-in period mice were randomly assigned to the LF
diet or the HF diet. The diets contained 10 kcal% fat or 45 kcal% fat in the form of palm oil
(based on D12450B and D12451; Research Diet Services, Wijk bij Duurstede, the …